construction and evaluation of a specific nonviral vector for ex-vivo gene correction of human β-globin gene defects
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Construction and cloning of a recombinant expression vector containing human Cd20 Gene for antibody therapy in Non-Hodgkin Lymphoma
ABSTRACT Introduction: Non-Hodgkin lymphoma (NHL) is a cancer that starts in lymphocytes. The main treatment for NHL is chemotherapy and radiation. Today immunotherapy is a promising therapeutic approach in the treatment of a variety cancers which is high specific unlike previous methods. Antibodies do not penetrate effectively into tumore tissues because of their large size. Whe...
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background: replacment of cd133+ cell’s β globin gene by using a gene targeting construct containing the β globin gene and essential elements for homologous recombination. materials and methods: pfbggt was amplified, then digested using the nhei and xhoi restriction enzymes, and finally, a 13.3 kb band (naked dna) was extracted from the agarose gel. biological activity of positive and negative ...
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Background: Tuberculosis (TB) is a major cause of death worldwide. Finding an effective vaccine against TB is the best way to control it. Several vaccines against this disease have been developed but none are completely protective. The aim of this study was to design and construct a cloning vector containing the Mycobacterium tuberculosis (M. tuberculosis) heat shock protein X (hspX). Metho...
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Objective: According to the characteristics of α/β-globin gene imbalance caused by α-globin gene accumulation in β-thalassemia, we designed two lentiviral vectors, that is, while we used the lentiviral to mediate full length human β-globin gene, we introduced the interference fragment of lentiviral α-globin gene to adjust the α/β chain ratio by increasing the expression of β-globin and decreasi...
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Gene transfer to vein grafts has therapeutic potential to prevent late graft failure; however, certain issues, including efficacy and safety, have hindered the clinical application of this treatment modality. Here, we report the successful and efficient gene transfer of plasmid DNA via ex vivo electroporation into veins as well as into vein grafts. Two approaches were used: one involved translu...
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عنوان ژورنال:
research in pharmaceutical sciencesجلد ۷، شماره ۵، صفحات ۰-۰
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